Keywords : Lactobacillus acidophilus
Inhibition of Uropathogenic Citrobacter Adhesion by Biosurfactants Extracted from Vaginal Lactobacillus acidophilus
Al- Anbar Medical Journal,
2012, Volume 10, Issue 1, Pages 59-67
Background and Objective: A study was conducted to determine the role of biosurfactants solution that was extracted from Lactobacillus acidophilus in the adhesion of uropathogenic Citrobacter on uroepithelial cells.
Methods: A total of 53 isolates of uropathogenic Citrobacter were isolated from female inpatients in Ramadi Maternity and Children Hospital, suffering from urinary tract infections. The age of patients ranges from 15 to 45 years. Lactobacillus acidophilus was isolated from healthy woman by vaginal swab and the biosurfactants were extracted from it. The adhesion of uropathogenic Citrobacter on uroepithelial cells were made as control. Another adhesion experiments was made in presence of biosurfactants solution or Lactobacillus acidophilus whole cells in the test tubes.
Results: The mean of uropathogenic Citrobacter adherent on uroepithelial cells in control tubes was 255.2 per 10 urpepithelial cells while the mean of uropathogenic Citrobacter adherent on uroepithelial cells in presence of L. acidophilus was 145.067 per 10 urpepithelial cells and the mean of uropathogenic Citrobacter adherent on uroepithelial cells in presence of Biosurfactants solution was 70.778 per 10 urpepithelial cells.
There was significant decreasing in numbers of uropathogenic Citrobacter which adhere on uroepithelial cells in presence of biosurfactants in the test solution, and the Lactobacillus acidophilus as whole bacteria can decrease the adhering number of uropathogenic Citrobacter in the test tubes
Conclusion: The biosurfactants solution has a potential effect to prevent the adhesion of uropathogenic Citrobacter on uroepithelial cells.
Key words: Biosurfactants, Lactobacillus acidophilus, Citrobacter, Adhesion