Al- Anbar Medical Journal

Correlation of Salivary Melatonin Levels and Many Periodontal Parameters in Cigarette Smokers with Periodontitis

Document Type : Original articles

Authors

1 Department of of periodontology, College of Dentistry, University of Baghdad, Iraq

2 Department of periodontology, College of Dentistry. University of Baghdad, Iraq.

Abstract

Background: Adults who have periodontitis are more likely to lose teeth, which affects their quality of life and causes masticatory dysfunction. Most people agree that smoking tobacco makes them more likely to get oral mucosal and periodontal diseases and makes the oral microbiome less balanced. Melatonin is a naturally occurring hormone that is released in a variety of organs.
 Objectives: To examine the relationship between smoking and salivary melatonin levels in periodontitis patients vs healthy people, and to establish a relationship between melatonin levels and periodontal parameters.
 Materials and Methods: The study was conducted at the College of Dentistry, University of Baghdad, Baghdad, Iraq. The study period was from February to May 2022. There were a total of 74 male subjects aged 35-55 years, and they were divided into four different groups; Group 1: a control group of non-smokers (n = 12), Group 2: a control group of smokers (n = 12), Group 3: a group of individuals with periodontitis (n = 25), Group 4: a group of individuals with both periodontitis and a record of smoking (n = 25). A whole, unstimulated sample of the participant's saliva (5 ml) was taken, and this was followed by an evaluation of clinical periodontal variables (probing pocket depth and clinical attachment level). To measure melatonin concentrations, saliva samples were biochemically analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA).
Results: Melatonin concentrations were shown to be lower in groups with periodontitis than in control groups, as well as lower in groups with smoking than in groups without smoking. It was found that there was a strong negative link between periodontal variables and the amount of melatonin in the saliva. 
Conclusion: According to this study, a reduced melatonin level was noticed in the salivary fluid of the smoker (healthy and periodontitis) groups in comparison with the non-smoker (healthy and periodontitis) groups. This suggests that active cigarette smoking had a retarding effect on the salivary levels of melatonin.  

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Main Subjects

References
[1]      L. Jia, N. Han, J. Du, L. Guo, Z. Luo, and Y. Liu, “Pathogenesis of important virulence factors of Porphyromonas gingivalis via toll-like receptors,” Front. Cell. Infect. Microbiol., vol. 9, p. 262, 2019.
[2]      F. D’Aiuto et al., “Evidence summary: The relationship between oral diseases and diabetes,” Br. Dent. J., vol. 222, no. 12, pp. 944–948, 2017.
[3]      C. Huang and G. Shi, “Smoking and microbiome in oral, airway, gut and some systemic diseases,” J. Transl. Med., vol. 17, no. 1, pp. 1–15, 2019.
[4]      F. R. M. Leite, G. G. Nascimento, F. Scheutz, and R. Lopez, “Effect of smoking on periodontitis: a systematic review and meta-regression,” Am. J. Prev. Med., vol. 54, no. 6, pp. 831–841, 2018.
[5]      I. Al-Assaf and M. Khazem, “Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria,” Iraqi J. Pharm. Sci. (P-ISSN 1683-3597 E-ISSN 2521-3512), vol. 30, no. 2, pp. 261–268, 2021.
[6]      S. Anil, “Study of the patterns of periodontal destruction in smokers with chronic periodontitis,” Indian J. Dent. Res., vol. 19, no. 2, p. 124, 2008.
[7]      M. A. El-Missiry, Z. M. A. El-Missiry, and A. I. Othman, “Melatonin is a potential adjuvant to improve clinical outcomes in individuals with obesity and diabetes with coexistence of Covid-19,” Eur. J. Pharmacol., vol. 882, p. 173329, 2020.
[8]      D. Acuña-Castroviejo et al., “Extrapineal melatonin: sources, regulation, and potential functions,” Cell. Mol. life Sci., vol. 71, pp. 2997–3025, 2014.
[9]      S. D. Paredes, A. Korkmaz, L. C. Manchester, D.-X. Tan, and R. J. Reiter, “Phytomelatonin: a review,” J. Exp. Bot., vol. 60, no. 1, pp. 57–69, 2009.
[10]    H. R. Hasan and J. A. Zainulabdeen, “Screening, Extraction, and Quantification of Melatonin in Waste of Some Plants,” Iraqi J. Sci., pp. 1874–1884, 2022.
[11]    A. Cutando, G. Gómez‐Moreno, C. Arana, D. Acuña‐Castroviejo, and R. J. Reiter, “Melatonin: potential functions in the oral cavity,” J. Periodontol., vol. 78, no. 6, pp. 1094–1102, 2007.
[12]    M. Gunata, H. Parlakpinar, and H. A. Acet, “Melatonin: A review of its potential functions and effects on neurological diseases,” Rev. Neurol. (Paris)., vol. 176, no. 3, pp. 148–165, 2020.
[13]    N. Suzuki, M. Somei, A. Seki, R. J. Reiter, and A. Hattori, “Novel bromomelatonin derivatives as potentially effective drugs to treat bone diseases,” J. Pineal Res., vol. 45, no. 3, pp. 229–234, 2008.
[14]    T. M. Balaji and S. R. Rao, “Salivary, Plasma, and Gingival Levels of Melatonin and TNF-α in Nonsmokers and Current Smokers with and without Periodontal Disease,” J. Contemp. Dent. Pract., vol. 21, no. 8, pp. 897–904, 2020.
[15]    K. Bjartveit and A. Tverdal, “Health consequences of smoking 1–4 cigarettes per day,” Tob. Control, vol. 14, no. 5, pp. 315–320, 2005.
[16]    M. S. Tonetti, H. Greenwell, and K. S. Kornman, “Staging and grading of periodontitis: Framework and proposal of a new classification and case definition,” J. Periodontol., vol. 89, pp. S159–S172, 2018.
[17]    M. Navazesh, “Methods for collecting saliva,” Ann. N. Y. Acad. Sci., vol. 694, no. 1, pp. 72–77, 1993.
[18]    T. Meulman et al., “Impact of supragingival therapy on subgingival microbial profile in smokers versus non-smokers with severe chronic periodontitis,” J. Oral Microbiol., vol. 4, no. 1, p. 8640, 2012.
[19]    L. R. A. Ameer and B. G. Ali, “Effects of light smoking on salivary levels of alkaline phosphatase and osteocalcin in chronic periodontitis patients,” J. Baghdad Coll. Dent., vol. 27, no. 2, pp. 110–114, 2015.
[20]    M. BinShabaib et al., “Clinical periodontal status and gingival crevicular fluid cytokine profile among cigarette-smokers, electronic-cigarette users and never-smokers,” Arch. Oral Biol., vol. 102, pp. 212–217, 2019.
[21]    K. Edman, K. Öhrn, B. Nordström, A. Holmlund, and D. Hellberg, “Trends over 30 years in the prevalence and severity of alveolar bone loss and the influence of smoking and socio‐economic factors–based on epidemiological surveys in Sweden 1983–2013,” Int. J. Dent. Hyg., vol. 13, no. 4, pp. 283–291, 2015.
[22]    J. Katz, R. M. Caudle, I. Bhattacharyya, C. M. Stewart, and D. M. Cohen, “Receptor for advanced glycation end product (RAGE) upregulation in human gingival fibroblasts incubated with nornicotine,” J. Periodontol., vol. 76, no. 7, pp. 1171–1174, 2005.
[23]    H. Abdolsamadi et al., “Reduction of melatonin level in patients with type II diabetes and periodontal diseases,” J. Dent. Res. Dent. Clin. Dent. Prospects, vol. 8, no. 3, p. 160, 2014.
[24]    R. S. Hassan and S. A. Salman, “Evaluation of Salivary Melatonin and Periodontal Parameters in Type II Diabetic Patients with Chronic Periodontitis: A Comparative Study.,” Indian J. Public Heal. Res. Dev., vol. 10, no. 10, 2019.
[25]    K. Lodhi, C. S. Saimbi, M. A. Khan, C. Nath, and R. Shukla, “Evaluation of melatonin levels in saliva in gingivitis and periodontitis cases: A pilot study,” Contemp. Clin. Dent., vol. 7, no. 4, p. 519, 2016.
[26]    R. J. Reiter, J. C. Mayo, D. Tan, R. M. Sainz, M. Alatorre‐Jimenez, and L. Qin, “Melatonin as an antioxidant: under promises but over delivers,” J. Pineal Res., vol. 61, no. 3, pp. 253–278, 2016.
[27]    N. Buduneli et al., “Effects of smoking and gingival inflammation on salivary antioxidant capacity,” J. Clin. Periodontol., vol. 33, no. 3, pp. 159–164, 2006.
[28]    Z. Donmez et al., “Evaluation of the antioxidant effects of melatonin on the larynx mucosa of rats exposed to environmental tobacco smoke,” Clin. Otolaryngol., vol. 41, no. 3, pp. 211–221, 2016.
[29]    R. Srinath, A. B. Acharya, and S. L. Thakur, “Salivary and gingival crevicular fluid melatonin in periodontal health and disease,” J. Periodontol., vol. 81, no. 2, pp. 277–283, 2010.
[30]    S. Najeeb, Z. Khurshid, S. Zohaib, and M. S. Zafar, “Therapeutic potential of melatonin in oral medicine and periodontology,” Kaohsiung J. Med. Sci., vol. 32, no. 8, pp. 391–396, 2016.
Al- Anbar Medical Journal
Volume 19, Issue 1
June 2023
Page 68-73
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