The Histological Changes on Liver and Spleen of Mice Treated with Alcoholic and Aquatic Extract of Cupressus sempervirens
Al- Anbar Medical Journal,
2012, Volume 10, Issue 2, Pages 51-57
AbstractBackground: Cupressus sempervirens has been widely cultivated as an ornamental tree for millennia away from its native range, mainly throughout the central and western Mediterranean region. It contains some active chemical constituents.
Objectives: The aim of the study was to investigate the effect of Cupressus sempervirens on the histological structure of liver and spleen.
Methods: For this purpose, thirty males Swiss albino mice were divided into five equal groups, the groups of aqueous and alcoholic extract of cupressus sempervirens were (350, 150, 0 mg/ml), six mice were used for each group. Then the animals were sacrificed and the histological change on liver and spleen was studied.
Results: Histopathology of liver in mice treated with 350 mg/ml alcoholic extract of Cupressus sempervirens showed aggregation of inflammation cells mainly neutrophil and lymphocyte, as dilated as congestion in the central vein , and the aquatic extract of 350 mg/mlCupressus sempervirens showed mononuclear cell infiltrate in the portal area together with amyloid deposition in sinusoid and multiple area of liver necrosis, while liver in mice treated with 150 mg/ml alcoholic extract of Cupressus sempervirens showed proliferation of kapffer cell and congestion of central vein , while at 150 mg/ml aquatic extract of Cupressus sempervirens showed proliferation of kapffer cell with double nucleated. Histopathology of spleen in mice treated with 350 mg/ml alcoholic and aquatic extract of Cupressus sempervirens showed severe amyloid deposition in red pulp and around white pulp, while spleen in mice treated with 150 mg/ml alcoholic extract of Cupressus sempervirens showed moderate periarterial hyper plasma, and a sectional view of 150 mg/ml aquatic extract of Cupressus sempervirens showed depletion of white pulp mainly neutrophil and macrophage.
Conclusions: from the result of this study it can be conclude that the high concentration of Cupressus sempervirens histopathological effect on liver and spleen of mice that induced with.
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